Dhurrin biosynthesis in sorghum
WebHere, we dissect the genetic control of leaf dhurrin content through a genome-wide association study (GWAS) using a panel of 700 diverse converted sorghum lines (conversion panel) previously subjected to pre-breeding and selected for short stature (∼1 m in height) and photoperiod insensitivity. WebKeywords: Dhurrin biosynthesis, GWAS, Genome, Genome evolution, Sorghum sudanese Pubmed ID: 36912984 DOI: 10.1007/s00122-023-04262-9. Abstract ...
Dhurrin biosynthesis in sorghum
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WebJan 27, 2024 · In the domesticated sorghum, dhurrin content was significantly higher compared to that in the wild sorghum and increased with stress and decreased with age whereas in wild sorghum the dhurrin content remained negligible. The key genes in dhurrin biosynthesis, CYP79A1, CYP71E1 and UGT85B1, were shown to be highly … WebIn sorghum, dhurrin is mainly known for acting as a nitrogen storage compound, an osmoprotectant, or a phytoanticipin [ 7 ]. Dhurrin is derived from L-tyrosine by three biosynthetic enzymes: two cytochrome P450, CYP79A1 and CYP71E1, and UDP-glycosyltransferase, UGT85B1.
WebDec 13, 2016 · Crucial for this function is the endogenous turnover of dhurrin for which putative pathways have been suggested but not confirmed. Results: In this study, the … WebThe purpose of our study is to estimate the contents of dhurrin together with its peripheral metabolites using an HPLC system and to profile gene expression patterns associated with dhurrin biosynthesis by quantitative RT-PCR (qRT-PCR) in 17 sorghum accessions provided by various resources, allowing us to investigate the correlations between the …
WebNov 14, 2016 · The dhurrin gene cluster in sorghum contains SbMATE2 and SbGST1, which are co-expressed with the biosynthetic genes. (a) genomic organisation of the … WebJan 1, 2016 · Dhu1 is aligned with genes involved in dhurrin biosynthesis (CYP79A1, CYP71E1, UGT85B1). Protein sequence variants found in CYP71E1 and UGT85B1 …
WebMar 29, 2024 · The cyanogenic glucoside, dhurrin, present in Sorghum bicolor is thought to have multiple functions, including in defence against herbivory. The hormone methyl jasmonate (MeJA) is also induced by herbivory and …
WebTo investigate whether dhurrin is induced in response to herbivore attack and also to the associated presence of MeJA, sorghum plants were either wounded or exogenous … can am 2 up seat removalWebAbstract. Dhurrin is the most abundant cyanogenic glucoside found in sorghum (Sorghum bicolor) where it plays a key role in chemical defense by releasing toxic hydrogen cyanide upon tissue disruption.Besides this well-established function, there is strong evidence that dhurrin plays additional roles, e.g. as a transport and storage form of nitrogen, released … can am3 fit in am3WebA cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450 – (V/I)KEX (L/F)R, FXPERF, and PFGXGRRXCXG – was applied. can a m4 bumper fit on a 525iWebFeb 26, 2024 · Sorghum is a C4 photosynthetic, drought resistant forage and grain plant species. Sorghum is cyanogenic in all tissues except for the mature seeds. The … fisher price puzzleWebNov 12, 2024 · In this study, RNA-seq was used to investigate the expression of genes involved in the biosynthesis, bio-activation and recycling of dhurrin in Sorghum bicolor. Genes involved in dhurrin biosynthesis were highly expressed in all young developing vegetative tissues (leaves, leaf sheath, roots, stems), tiller buds and imbibing seeds and … fisher price push walkerWebIn vitro biosynthesis of dhurrin has been constructed in both microsomes recovered from Sorghum bicolor seedlings and in micelles. D'après les études in vitro et ex vivo réalisées sur des microsomes hépatiques félins, il est possible … fisher price push tricycleWebFeb 1, 2024 · Dhurrin biosynthesis in the presence of different NADESs. A) Etiolated sorghum seedlings used for preparation of microsomes. B) Tyrosine conversion assay … can am 450 outlander 2021